The most accurate hot start DNA polymerase on the market featuring extreme specificity and improved performance.
Thermo Scientific Phusion Hot Start II High Fidelity DNA Polymerase is highly accurate hot start DNA polymerase. It combines the Phusion DNA Polymerase and a reversibly bound, specific Affibody ligand. Affibody inhibits the DNA polymerase activity at room temperature and thus prevents the amplification of nonspecific products.
The Affibody ligand also inhibits the 3´→5´ exonuclease activity of the polymerase, preventing degradation of primers and template DNA during reaction set up. At polymerization temperatures, the ligand is released, rendering the polymerase fully active. Phusion Hot Start II DNA Polymerase does not require a separate activation step in the PCR protocol as it is immediately reactivated at high temperatures.
Phusion Green Hot Start II High-Fidelity DNA Polymerase is a combination of Phusion Hot Start II DNA Polymerase and 5X Phusion Green Buffers. The buffers includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with Phusion Hot Start II DNA Polymerase performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.
Phusion Hot Start II High-Fidelity PCR Master Mixes are convenient 2X mixes designed to minimize the number of pipetting steps. The master mixes contain Phusion Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Only template and primers need to be added to the PCR reaction.
Phusion and Phusion Green Hot Start II High-Fidelity DNA Polymerase
Phusion and Phusion Green Hot Start II High-Fidelity PCR Master Mix
F-537S | Phusion Green Hot Start II High-Fidelity DNA Polymerase (2 U/µL) | 100 units |
---|---|---|
F-537L | Phusion Green Hot Start II High-Fidelity DNA Polymerase (2 U/µL) | 500 units |
F-566S | Phusion Green Hot Start II High-Fidelity PCR Master Mix | 100 x 50 µL rxns |
F-566L | Phusion Green Hot Start II High-Fidelity PCR Master Mix | 500 x 50 µL rxns |
F-549S | Phusion Hot Start II High-Fidelity DNA Polymerase | 100 units |
F-549L | Phusion Hot Start II High-Fidelity DNA Polymerase | 500 units |
F-565S | Phusion Hot Start II High-Fidelity PCR Master Mix | 100 x 50 µL rxns |
F-565L | Phusion Hot Start II High-Fidelity PCR Master Mix | 500 x 50 µL rxns |
The most accurate hot start DNA polymerase on the market featuring extreme specificity and improved performance.
Thermo Scientific Phusion Hot Start II High Fidelity DNA Polymerase is highly accurate hot start DNA polymerase. It combines the Phusion DNA Polymerase and a reversibly bound, specific Affibody ligand. Affibody inhibits the DNA polymerase activity at room temperature and thus prevents the amplification of nonspecific products.
The Affibody ligand also inhibits the 3´→5´ exonuclease activity of the polymerase, preventing degradation of primers and template DNA during reaction set up. At polymerization temperatures, the ligand is released, rendering the polymerase fully active. Phusion Hot Start II DNA Polymerase does not require a separate activation step in the PCR protocol as it is immediately reactivated at high temperatures.
Phusion Green Hot Start II High-Fidelity DNA Polymerase is a combination of Phusion Hot Start II DNA Polymerase and 5X Phusion Green Buffers. The buffers includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with Phusion Hot Start II DNA Polymerase performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.
Phusion Hot Start II High-Fidelity PCR Master Mixes are convenient 2X mixes designed to minimize the number of pipetting steps. The master mixes contain Phusion Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Only template and primers need to be added to the PCR reaction.
Phusion and Phusion Green Hot Start II High-Fidelity DNA Polymerase
Phusion and Phusion Green Hot Start II High-Fidelity PCR Master Mix
F-537S | Phusion Green Hot Start II High-Fidelity DNA Polymerase (2 U/µL) | 100 units |
---|---|---|
F-537L | Phusion Green Hot Start II High-Fidelity DNA Polymerase (2 U/µL) | 500 units |
F-566S | Phusion Green Hot Start II High-Fidelity PCR Master Mix | 100 x 50 µL rxns |
F-566L | Phusion Green Hot Start II High-Fidelity PCR Master Mix | 500 x 50 µL rxns |
F-549S | Phusion Hot Start II High-Fidelity DNA Polymerase | 100 units |
F-549L | Phusion Hot Start II High-Fidelity DNA Polymerase | 500 units |
F-565S | Phusion Hot Start II High-Fidelity PCR Master Mix | 100 x 50 µL rxns |
F-565L | Phusion Hot Start II High-Fidelity PCR Master Mix | 500 x 50 µL rxns |
The most accurate hot start DNA polymerase on the market featuring extreme specificity and improved performance.
Thermo Scientific Phusion Hot Start II High Fidelity DNA Polymerase is highly accurate hot start DNA polymerase. It combines the Phusion DNA Polymerase and a reversibly bound, specific Affibody ligand. Affibody inhibits the DNA polymerase activity at room temperature and thus prevents the amplification of nonspecific products.
The Affibody ligand also inhibits the 3´→5´ exonuclease activity of the polymerase, preventing degradation of primers and template DNA during reaction set up. At polymerization temperatures, the ligand is released, rendering the polymerase fully active. Phusion Hot Start II DNA Polymerase does not require a separate activation step in the PCR protocol as it is immediately reactivated at high temperatures.
Phusion Green Hot Start II High-Fidelity DNA Polymerase is a combination of Phusion Hot Start II DNA Polymerase and 5X Phusion Green Buffers. The buffers includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with Phusion Hot Start II DNA Polymerase performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.
Phusion Hot Start II High-Fidelity PCR Master Mixes are convenient 2X mixes designed to minimize the number of pipetting steps. The master mixes contain Phusion Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Only template and primers need to be added to the PCR reaction.
Phusion and Phusion Green Hot Start II High-Fidelity DNA Polymerase
Phusion and Phusion Green Hot Start II High-Fidelity PCR Master Mix
F-537S | Phusion Green Hot Start II High-Fidelity DNA Polymerase (2 U/µL) | 100 units |
---|---|---|
F-537L | Phusion Green Hot Start II High-Fidelity DNA Polymerase (2 U/µL) | 500 units |
F-566S | Phusion Green Hot Start II High-Fidelity PCR Master Mix | 100 x 50 µL rxns |
F-566L | Phusion Green Hot Start II High-Fidelity PCR Master Mix | 500 x 50 µL rxns |
F-549S | Phusion Hot Start II High-Fidelity DNA Polymerase | 100 units |
F-549L | Phusion Hot Start II High-Fidelity DNA Polymerase | 500 units |
F-565S | Phusion Hot Start II High-Fidelity PCR Master Mix | 100 x 50 µL rxns |
F-565L | Phusion Hot Start II High-Fidelity PCR Master Mix | 500 x 50 µL rxns |