A novel hot start DNA Polymerase for routine and high throughput PCR applications requiring speed and specifity.
Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It outperforms every Taq-based hot start polymerase. Phire Hot Start II DNA Polymerase is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields. These features are achieved through advanced protein engineering of the polymerase. It incorporates a unique double-stranded DNA binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase.
The hot start modification of Phire Hot Start II DNA Polymerase is based on the Affibody inactivation technology. This technology increases the specificity of the PCR reaction with no additional time required for initial activation of the enzyme.
Phire Green format is a combination of Phire Hot Start II DNA Polymerase and 5X Phire Green Buffer. The buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.
Phire Hot Start II PCR Master Mixes are convenient 2X mixes designed to minimize the number of pipetting steps. The master mixes contain Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Only template and primers need to be added to PCR reaction.
Phire or Phire Green Hot Start II DNA Polymerase
Phire or Phire Green Hot Start II PCR Master Mix
Phusion and Phusion Green Hot Start II High-Fidelity PCR Master Mix
F-124S | Phire Green Hot Start II DNA Polymerase | 200 x 50 µl rxns |
---|---|---|
F-124L | Phire Green Hot Start II DNA Polymerase | 1000 x 50 µl rxns |
F-126S | Phire Green Hot Start II PCR Master Mix | 200 x 50 µl rxns |
F-126L | Phire Green Hot Start II PCR Master Mix | 1000 x 50 µl rxns |
F-122S | Phire Hot Start II DNA Polymerase | 200 x 50 µl rxns |
F-122L | Phire Hot Start II DNA Polymerase | 1000 x 50 µl rxns |
F-125S | Phire Hot Start II PCR Master Mix | 200 x 50 µl rxns |
F-125L | Phire Hot Start II PCR Master Mix | 1000 x 50 µl rxns |
A novel hot start DNA Polymerase for routine and high throughput PCR applications requiring speed and specifity.
Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It outperforms every Taq-based hot start polymerase. Phire Hot Start II DNA Polymerase is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields. These features are achieved through advanced protein engineering of the polymerase. It incorporates a unique double-stranded DNA binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase.
The hot start modification of Phire Hot Start II DNA Polymerase is based on the Affibody inactivation technology. This technology increases the specificity of the PCR reaction with no additional time required for initial activation of the enzyme.
Phire Green format is a combination of Phire Hot Start II DNA Polymerase and 5X Phire Green Buffer. The buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.
Phire Hot Start II PCR Master Mixes are convenient 2X mixes designed to minimize the number of pipetting steps. The master mixes contain Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Only template and primers need to be added to PCR reaction.
Phire or Phire Green Hot Start II DNA Polymerase
Phire or Phire Green Hot Start II PCR Master Mix
Phusion and Phusion Green Hot Start II High-Fidelity PCR Master Mix
F-124S | Phire Green Hot Start II DNA Polymerase | 200 x 50 µl rxns |
---|---|---|
F-124L | Phire Green Hot Start II DNA Polymerase | 1000 x 50 µl rxns |
F-126S | Phire Green Hot Start II PCR Master Mix | 200 x 50 µl rxns |
F-126L | Phire Green Hot Start II PCR Master Mix | 1000 x 50 µl rxns |
F-122S | Phire Hot Start II DNA Polymerase | 200 x 50 µl rxns |
F-122L | Phire Hot Start II DNA Polymerase | 1000 x 50 µl rxns |
F-125S | Phire Hot Start II PCR Master Mix | 200 x 50 µl rxns |
F-125L | Phire Hot Start II PCR Master Mix | 1000 x 50 µl rxns |
A novel hot start DNA Polymerase for routine and high throughput PCR applications requiring speed and specifity.
Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It outperforms every Taq-based hot start polymerase. Phire Hot Start II DNA Polymerase is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields. These features are achieved through advanced protein engineering of the polymerase. It incorporates a unique double-stranded DNA binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase.
The hot start modification of Phire Hot Start II DNA Polymerase is based on the Affibody inactivation technology. This technology increases the specificity of the PCR reaction with no additional time required for initial activation of the enzyme.
Phire Green format is a combination of Phire Hot Start II DNA Polymerase and 5X Phire Green Buffer. The buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.
Phire Hot Start II PCR Master Mixes are convenient 2X mixes designed to minimize the number of pipetting steps. The master mixes contain Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2. Only template and primers need to be added to PCR reaction.
Phire or Phire Green Hot Start II DNA Polymerase
Phire or Phire Green Hot Start II PCR Master Mix
Phusion and Phusion Green Hot Start II High-Fidelity PCR Master Mix
F-124S | Phire Green Hot Start II DNA Polymerase | 200 x 50 µl rxns |
---|---|---|
F-124L | Phire Green Hot Start II DNA Polymerase | 1000 x 50 µl rxns |
F-126S | Phire Green Hot Start II PCR Master Mix | 200 x 50 µl rxns |
F-126L | Phire Green Hot Start II PCR Master Mix | 1000 x 50 µl rxns |
F-122S | Phire Hot Start II DNA Polymerase | 200 x 50 µl rxns |
F-122L | Phire Hot Start II DNA Polymerase | 1000 x 50 µl rxns |
F-125S | Phire Hot Start II PCR Master Mix | 200 x 50 µl rxns |
F-125L | Phire Hot Start II PCR Master Mix | 1000 x 50 µl rxns |